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Protein Assay Kit Thermo Fisher Scientific 22662 Piercetm Silver Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Protein Silver Staining Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Protein Quickly Silver Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Protein Silver Staining Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Protein Silver Stain Kit P0017s, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Pierce Silver Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) <t>Silver</t> <t>staining</t> of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) <t>Protein-protein</t> interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex
Protein Silver Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein silver staining kit/product/Beyotime
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Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) Silver staining of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) Protein-protein interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex

Journal: Cancer Cell International

Article Title: A LINC00472-encoded polypeptide impedes migration and proliferation through modulation of the HDAC2/SP1 axis in non-small cell lung cancer cells

doi: 10.1186/s12935-025-03901-z

Figure Lengend Snippet: Interaction between the LINC00472-ORF polypeptide with HDAC2/SP1 axis. ( A - E ) Proteomic screening and interaction network analysis of LINC00472-ORF-associated proteins. ( A ) Co-immunoprecipitation (Co-IP) validation of Flag-tagged LINC00472-ORF polypeptide in A549 cells using anti-Flag antibody. ( B ) Silver staining of Co-IP eluates from IgG control and Flag-IP groups. ( C ) Venn diagram identifying 384 unique proteins enriched in Flag-IP eluates. ( D and E ) GO enrichment analysis and KEGG pathway analysis of the 384 proteins. ( F ) Protein-protein interaction (PPI) network of the identified proteins generated via STRING database, highlighting SP1 as a central hub. ( G ) Schematic of the protein-protein docking pose of the polypeptide/HDAC2/SP1 complex

Article Snippet: Immunoprecipitated proteins were separated by 12% SDS-PAGE and visualized using the Protein Silver Staining Kit (Servicebio) according to the manufacturer’s protocol.

Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Biomarker Discovery, Silver Staining, Control, Generated